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Carbon isotope signature variability among cultured microalgae: Influence of species, nutrients and growth

  • Andreas Brutemark
  • Elin Lindehoff
  • Edna Graneli
  • Wilhelm Granéli
Publishing year: 2009
Language: English
Pages: 98-105
Publication/Series: Journal of Experimental Marine Biology and Ecology
Volume: 372
Issue: 1-2
Document type: Journal article
Publisher: Elsevier

Abstract english

In this study we have investigated whether the carbon isotopic signature differs between different groups and species of marine phytoplankton depending on growth phase, nutrient conditions and salinity. The 15 investigated algal species, representing the Bacillariophyceae, Chlorophyceae, Cryptophyceae, Cyanophyceae, Dinophyceae and Haptophyceae classes were grown in batch monocultures and analysed for delta C-13 in both exponential and stationary phase. For all the cultured species, delta C-13 signatures ranged from -23.5 parts per thousand (Imantonia sp.) to - 12.3 parts per thousand (Nodulania spumigena) in the exponential phase and from - 18.8 parts per thousand (Amphidinium carterae) to - 8.0 parts per thousand (Anabaena lemmermannii) in the stationary phase. Three species (Dunaliella tertiolecta, Rhodomonas sp.. Heterocapsa triquetra) were also grown under nutrient sufficient and nitrogen or phosphorus deficient conditions. Nitrogen limitation resulted in a more negative delta C-13 signature, whereas no effect could be observed during phosphorus limitation compared to nutrient sufficient conditions. Growth of Prymnesium parvum in two different salinities resulted in a more negative delta C-13 signature in the 26 parts per thousand-media compared to growth in 7 parts per thousand-media. Our results show that the carbon isotopic signature of phytoplankton may be affected by salinity, differ among different phytoplankton species, between exponential and stationary phase, as well as between nutrient treatments. (C) 2009 Elsevier B.V. All rights reserved.


  • Ecology
  • Stationary phase
  • Nutrients
  • Isotope
  • Exponential phase
  • Culture
  • Algae
  • Carbon


  • ISSN: 0022-0981
Wilhelm Granéli
E-mail: wilhelm [dot] graneli [at] biol [dot] lu [dot] se

Professor emeritus

Aquatic ecology

+46 70 279 82 34