Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.

Author:
  • Magnus Ellström
  • Firoz Shah
  • Tomas Johansson
  • Dag Ahrén
  • Per Persson
  • Anders Tunlid
Publishing year: 2015
Language: English
Publication/Series: FEMS microbiology ecology
Volume: 91
Issue: 4
Document type: Journal article
Publisher: Elsevier

Abstract english

The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases, and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon-starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils.

Keywords

  • Biological Sciences
  • Earth and Related Environmental Sciences

Other

Published
  • MICCS - Molecular Interactions Controlling soil Carbon Sequestration
  • Microbial Ecology
  • ISSN: 1574-6941
Tomas Johansson
E-mail: tomas [dot] johansson [at] biol [dot] lu [dot] se

Research engineer

MEMEG

+46 46 222 45 49

+46 70 957 18 04

E-A252

Sölvegatan 37, Lund

50

Health and safety representative

Evolutionary ecology

50