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Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus Trametes versicolor PRL 572

Author:
  • Tomas Johansson
  • Per-Olof Nyman
  • Daniel Cullen
Publishing year: 2002
Language: English
Pages: 2077-2080
Publication/Series: Applied and Environmental Microbiology
Volume: 68
Issue: 4
Document type: Journal article
Publisher: American Society for Microbiology

Abstract english

A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology.

Keywords

  • Biological Sciences

Other

Published
  • Microbial Ecology
  • ISSN: 0099-2240
Tomas Johansson
E-mail: tomas [dot] johansson [at] biol [dot] lu [dot] se

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