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Differential polyamine analogue effects in four human breast cancer cell lines

Author:
  • Martina Holst
  • Benjamin Frydman
  • Laurance J. Marton
  • Stina Oredsson
Publishing year: 2006
Language: English
Pages: 71-81
Publication/Series: Toxicology
Volume: 223
Issue: 1-2
Document type: Journal article
Publisher: Elsevier

Abstract english

Polyamine analogues have demonstrated anti-tumour activity in a number of solid tumour models. In the present study we

compared the cytotoxicities of three polyamine analogues against four breast cancer cell lines. All cell lines are derived from tumours of women with breast cancer and, although we are sampling just a small number of tumours, they represent a spectrum of the genetic plethora of breast cancers. Cytotoxicity, over a dose range from 0.1 to 100 microM, was evaluated with three different

cytotoxicity assays performed in 96-well plates. Comparing the effects of the analogues on polyamine pools with data from the cytotoxicity assays indicates that there was not a direct correlation between polyamine pool depletion and cytotoxicity. Flow cytometry was used to investigate analogue-induced cell death as measured by the appearance of a sub-G1 peak. Induction of cell death by the analogues differed in the cell lines, however, cell death when induced was apoptotic, as demonstrated by detection of

apoptotic bodies with immunofluorescence microscopy of propidium iodide-stained nuclei. Comparing the flow cytometry-derived data and the data from the cytotoxicity assays reveals that the analogues exert their effects by inhibiting cell growth and/or inducing cell death.

Keywords

  • Zoology
  • Polyamine analogues
  • Human breast cancer
  • CGC-11158
  • CGC-11047
  • CGC-11093
  • Cell death

Other

Published
  • ISSN: 0300-483X
Stina Oredsson
E-mail: stina [dot] oredsson [at] biol [dot] lu [dot] se

Professor

Functional zoology

+46 46 222 94 97

B-C208

4

Principal investigator

LUCC - Lund University Cancer Centre

Research group

Animal Physiology

Projects

Cell proliferation

Doctoral students and postdocs

PhD Students, main supervisor

Wendy Soria Sotillo

PhD Students, assistant supervisor

Atena Malakpour Permlid