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Importance of polyamines in cell cycle kinetics as studied in a transgenic system.

Author:
  • Sima Nasizadeh
  • Louise Myhre
  • Lena Thiman
  • Kersti Alm
  • Stina Oredsson
  • Lo Persson
Publishing year: 2005
Language: English
Pages: 254-264
Publication/Series: Experimental Cell Research
Volume: 308
Issue: May 26
Document type: Journal article
Publisher: Academic Press

Abstract english

Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present study, we have for the first time used a transgenic cell system to analyze the importance of polyamines in cell growth. We have earlier shown that expression of trypanosomal ODC in an ODC-deficient variant of CHO cells (C55.7) supported growth of these otherwise polyamine auxotrophic cells. However, one of the transgenic cell lines grew much slower than the others. As shown in the present study, the level of ODC activity was much lower in these cells, and that was reflected in a reduction of cellular polyamine levels. Analysis of cell cycle kinetics revealed that reduction of growth was correlated to prolongation of the G(1), S, and G(2) + M phases in the cells. Providing exogenous putrescine to the cells resulted in a normalization of polyamine levels as well as cell cycle kinetics indicating a causal relationship.

Keywords

  • Pharmacology and Toxicology
  • Basic Medicine
  • polyamines
  • ornithine decarboxylase
  • trypanosomal
  • cyclins
  • cell growth
  • cell cycle

Other

Published
  • Biogenic Amines
  • ISSN: 1090-2422
Stina Oredsson
E-mail: stina [dot] oredsson [at] biol [dot] lu [dot] se

Professor

Functional zoology

+46 46 222 94 97

B-C208

4

Principal investigator

LUCC - Lund University Cancer Centre

Research group

Animal Physiology

Projects

Cell proliferation

Doctoral students and postdocs

PhD Students, main supervisor

Wendy Soria Sotillo

PhD Students, assistant supervisor

Atena Malakpour Permlid