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Estimating the variation in S phase duration from flow cytometric histograms.

Author:
  • Sara Larsson
  • Tobias Rydén
  • Ulla Holst
  • Stina Oredsson
  • Maria C Johansson
Publishing year: 2008
Language: English
Pages: 40-49
Publication/Series: Mathematical Biosciences
Volume: 213
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.

Keywords

  • Cancer and Oncology
  • Branching process
  • Cell cycle progression
  • S phase duration
  • Flow cytometry

Other

Published
  • ISSN: 0025-5564
Stina Oredsson
E-mail: stina [dot] oredsson [at] biol [dot] lu [dot] se

Professor

Functional zoology

+46 46 222 94 97

B-C208

4

Principal investigator

LUCC - Lund University Cancer Centre

Research group

Animal Physiology

Projects

Cell proliferation

Doctoral students and postdocs

PhD Students, main supervisor

Wendy Soria Sotillo

PhD Students, assistant supervisor

Atena Malakpour Permlid