Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Effect of polyamine deficiency on proteins involved in Okazaki fragment maturation.

Author:
  • Veronica Johansson
  • Maria Falck Miniotis
  • Cecilia Hegardt
  • Göran B Jönsson
  • Johan Staaf
  • Pia Berntsson
  • Stina Oredsson
  • Kersti Alm
Publishing year: 2008
Language: English
Pages: 1467-1477
Publication/Series: Cell Biology International
Volume: 32
Document type: Journal article
Publisher: Elsevier

Abstract english

Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion sensitivity, something that can be useful for cancer treatment with polyamine analogues. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis.

Keywords

  • Cancer and Oncology
  • CGP 48664
  • DENSPM
  • human breast cancer
  • FEN1
  • DNA ligase I

Other

Published
  • Molecular Neurogenetics
  • ISSN: 1095-8355
Stina Oredsson
E-mail: stina [dot] oredsson [at] biol [dot] lu [dot] se

Professor

Functional zoology

+46 46 222 94 97

B-C208

4

Principal investigator

LUCC - Lund University Cancer Centre

Research group

Animal Physiology

Projects

Cell proliferation

Doctoral students and postdocs

PhD Students, main supervisor

Wendy Soria Sotillo

PhD Students, assistant supervisor

Atena Malakpour Permlid