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Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples

Author:
  • Talatu Tende
  • Bengt Hansson
  • Ulf Ottosson
  • Staffan Bensch
Publishing year: 2014
Language: English
Pages: 351-358
Publication/Series: Current Zoology
Volume: 60
Issue: 3
Document type: Journal article
Publisher: Current Zoology

Abstract english

Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that employed different media for the storage of faeces, although patterns of success were not fully consistent among different media, ethanol storage was scored highest in the majority of these tests.

Keywords

  • Biological Sciences
  • Amplification success
  • Faecal sample DNA
  • Lion Panthera leo
  • PCR
  • Preservation medium

Other

Published
  • BECC
  • Conservation of threatened populations
  • Molecular Ecology and Evolution Lab
  • ISSN: 1674-5507
Staffan Bensch
E-mail: staffan [dot] bensch [at] biol [dot] lu [dot] se

Professor

MEMEG

+46 46 222 42 92

E-C213

Sölvegatan 37, Lund

50

Head of unit

MEMEG

+46 46 222 42 92

E-C213

Sölvegatan 37, Lund

50

Research group

Molecular Ecology and Evolution Lab

Projects

Doctoral students and postdocs

PhD students, main supervisor

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