From the 3 – 7 December, 2018, LP3 host the practical postgraduate course Protein Factories
In this course the participants will get hands-on experience of protein production and purification using bacterial and insect cell-based expression systems (baculovirus expression vector system (BEVS)). The course presents methods to express genes, which have products that are known, unknown or not well characterized, and to analyze the gene products. Theories behind the methods are discussed and experiments are performed. The methods to be presented include: optimization of recombinant gene expression in Escherichia coli and in the BEVS; affinity purification of tagged proteins; using proteases to for the removal of protein fusion tags; generation and analysis of gene fusions; molecular cloning; metabolic engineering; seleno-methionine incorporation for MAD phasing; 2H-, 13C-, 15N-labeling for NMR studies; production of perdueterated proteins for neutron-scattering experiments. Strategies for improving production of "difficult" proteins will be presented. The lectures will cover the current status of cell-based protein production systems, and theoretical aspects of the methodology.