LP3 - Lund Protein Production Platform
The services available include
- Cloning - design of a vector construct, ordering of a synthetic gene, cloning and plasmid preparation.
- Protein production in bacterial (E. coli) or eukaryotic (insect) cells.
- Microbiological growth monitoring (Bioscreen C).
- Protein labeling (seleno-methionine incorporation, labeling with stable isotopes (2H, 13C, 15N), biotinylation, phosphorylation).
- Protein purification using state of the art chromatography equipment.
- Development of expression and purification protocols.
- High-throughput protein crystallization.
LP3 works as an intellectual hub for exchange of scientific ideas and for dissemination and assimilation of new methodologies for protein production, purification and analysis. The service facility is integrated with research training and development of skills in experimental protein science for PhD students and postdocs.
What are the costs?
In view of the customised nature of the services provided it is necessary to obtain a quotation from LP3 for the desired work.
A price list for protein crystallisation and characterisation can be found here.
For access to additional protein characterisation methods (CD, MS, NMR, etc) we can help you come in contact with the appropriate experts. A list of all available infrastructures at Lund University can be found at the Faculty of Medicine's website.
LP3 is one of several protein production facilities in Sweden that form the Protein Production Network Sweden (PPNS), each with its own specialties and focus. PPNS is an informal network formed in 2013 between protein production facilities at Swedish universities. The overall aim is to make competence and protein production methodologies available to the Swedish academic life science community and to make best use of our available resources.
The DEuteration and MAcromolecular Xtallization (DEMAX) platform of the European Spallation Source ERIC (ESS) moved into Biology building A at Lund University and is co-localized with the LP3. DEMAX and LP3 are collaborating to coordinate their efforts to develop cost-effective production of deuterated biomaterials (lipids and proteins) for neutron-based methods such as protein crystallography, neutron reflectometry, and small angle neutron scattering.
LP3 is also engaged in the European association Core Technologies for the Life Sciences, in the P4EU (Protein Production and Purification Partnership in Europe) and in ARBRE-MOBIEU (Association of Resources for Biophysical Research in Europe and Molecular Biophysics in Europe). Since 2019, LP3 is also affiliated with EATRIS, the European infrastructure for translational medicine and EATRIS small molecule platform.
The LP3 labs are located on floor 1 in Biology building A, Sölvegatan 35, Lund.
Lund Protein Production Platform
Biology Building A
+46 46 2227785
lp3 [at] biol [dot] lu [dot] se
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LP3 acknowledges generous financial support from Lund University and BioCARE (A Strategic Research Area at Lund University).
LP3 and the former crystallization facility acknowledge past generous financial support from Lund University, Knut and Alice Wallenberg Foundation (SWEGENE programme), the Erik & Maja Lundqvist Foundation, the Carl Tesdorpf Foundation and the Swedish Research Council.