Fragmentation of DNA and RNA
Covaris M220 Focused-ultrasonicator™ is designed for Next-Gen Sequencing applications requiring DNA fragment sizes between 150bp and 5kb. Compact size and ease-of-use make the M220 Focused-ultrasonicator the ideal DNA shearing solution.
Sample guidelines are up to 5 µg of purified DNA. A regular Tris-EDTA, pH 8.0 buffer is recommended and genomic DNA quality should be > 10 kb. For lower quality DNA, Covaris recommends setting up a time-dose response experiment for determining appropriate treatment times.
The following sample vials are kept in stock, but for more options visit the Covaris website:
- 130 ul sample volume – from 150bp to 1,500bp fragments (microTUBE AFA Fiber Snap-Cap)
- 50 µl sample volume – from 150bp to 800bp fragments (microTUBE-50 AFA Fiber Screw-Cap)
For fragmentation on Covaris M220 there is cost for tubes used (one tube per sample). The cost for AFA-tubes is approx. 70SEK each. On top of that, we add a 30% service charge (as of 2019-04) on the total to cover for other consumables and service contracts. For all users “overhead” charges are additionally applied. For users outside Lund University we also need to charge for labor on an hourly basis.
Sample form – Covaris DNA fragmentation
A Sample Form (see FragmentationForm; .xlsx; 30 kB) for fragmentation of DNA is required providing information about the client, invoicing information and samples provided. Please label your tube(s) numerically in accordance.
The sequencing Facility is situated at Department of Biology, in the Ecology Building, Sölvegatan 37 on Floor 2 (staircase A).
For inquiries about Illumina MiSeq sequencing, analyses on Agilent BioAnalyzer and/or fragmentation of DNA/RNA on Covaris, contact:
Telephone: +46 46-222 45 49
Mobile: +46 709-57 18 04
E-mail: Tomas [dot] Johansson [at] biol [dot] lu [dot] se
For inquiries about ABI Sanger Sequencing and Fragment Analysis contact:
Telephone: +46 46-222 08 70
Mobile: +46 72-203 33 56
E-mail: Anna [dot] Sterngren [at] biol [dot] lu [dot] se
The facility acknowledges generous financial support during the years from the Knut and Alice Wallenberg Foundation (SWEGENE programme), the Crafoord Foundation and the Faculty of Science, Lund University.