As a registered Illumina user at my.illumina.com you will also get access to the Illumina BaseSpace, which is a cloud-based storage of sequencing data. From there the user can download compressed fastq files and, if needed, raw data. The DNA Sequencing Facility keeps a local backup of all data. In Illumina BaseSpace users can perform various kind of standard analyses of their next generation sequencing data with the help of BaseSpace apps. Many of these apps are wrappers for commonly used bioinformatic software.
However, in the majority of cases, users download the fastq files and perform their own analyses. A bioinformatic analysis can take considerable time. The DNA Sequencing Facility always encourage customers to take into account the costs of bioinformatics in their projects before they actually starts.
The DNA Sequencing Facility is happy to give advice on how to process and analyze data, especially in the fields of genome sequencing, 16S and 18S rRNA gene amplicon sequencing, gene prediction, annotation, gene expression and variant calling. If further assistance is needed the facility charge a reduced fee per hour.
The rate for bioinformatic support is 300 SEK per hour and is a subsidized prize for faculty staff only. Minimum charge is for two hours. For all users “overhead” charges will be applied.
The sequencing Facility is situated at Sölvegatan 37, in the Ecology Building on Floor 2 (stair case A)
For inquiries about Illumina MiSeq sequencing and analyses on Agilent BioAnalyzer contact:
Telephone: +46 46-222 45 49
Mobile: +46 709-57 18 04
E-mail: Tomas [dot] Johansson [at] biol [dot] lu [dot] se
For inquiries about ABI Sanger sequencing contact:
Telephone: +46 46-222 08 70
Mobile: +46 72-203 33 56
E-mail: Anna [dot] Sterngren [at] biol [dot] lu [dot] se
For inquiries about Bioinformatics contact:
Telephone: +46 46-222 94 19
E-mail: Bjorn [dot] Canback [at] biol [dot] lu [dot] se
The facility acknowledges generous financial support during the years from the Knut and Alice Wallenberg Foundation (SWEGENE programme), the Crafoord Foundation and the Faculty of Science, Lund University.