DNA Sequencing Facility
The sequencing facility consists of two units, one for traditional Sanger DNA sequencing and one for Next-Generation DNA sequencing (NGS). The facility also offers library preparation for NGS according to best practices and in accordance with rigorous quality standards. Our staff have expertise in most aspects of DNA sequence analyses and can offer help and advice to both experienced and less experienced users.
The services available are
- Sanger DNA sequencing and Fragment Analysis on Applied BioSystem 3500 Genetic Analyzer (8-capillary)
- Next-Generation Sequencing (NGS) on Illumina MiSeq and including library construction
- Quality checking/assessment of DNA and RNA using Agilent 2100 BioAnalyzer and fluorescent quantification (PicoGreen/RiboGreen)
- DNA and RNA Fragmentation on Covaris Focused-ultrasonicator
- Guidelines and support for sample collection, experimental designs and choice of protocol
- For any bioinformatic issues we refer to the National Bioinformatics Infrastructure (NBIS) node at our department.
- Applied BioSystem 3500 Genetic Fragment Analyzer
- Illumina MiSeq
- Agilent 2100 BioAnalyzer
- NanoDrop 2000c
- QuantiFlour ST fluorometer
- Covaris M220 Focused-ultrasonicator
- DigiLab HydroShear Plus
- Qiagen TissueLyzer II
- CASY Innovatis Cell/Bead counter
- Applied Biosystems 2720 Thermal Cyclers
The sequencing Facility is situated at Department of Biology, in the Ecology Building, Sölvegatan 37 on Floor 2 (staircase A).
For inquiries about Illumina MiSeq sequencing, analyses on Agilent BioAnalyzer and/or fragmentation of DNA/RNA on Covaris, contact:
Telephone: +46 46-222 45 49
Mobile: +46 709-57 18 04
E-mail: Tomas [dot] Johansson [at] biol [dot] lu [dot] se
For inquiries about ABI Sanger Sequencing and Fragment Analysis contact:
Telephone: +46 46-222 08 70
Mobile: +46 72-203 33 56
E-mail: Anna [dot] Sterngren [at] biol [dot] lu [dot] se
The facility acknowledges generous financial support during the years from the Knut and Alice Wallenberg Foundation (SWEGENE programme), the Crafoord Foundation and the Faculty of Science, Lund University.