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High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy

Author:
  • Johan Vestergren
  • Andrea G. Vincent
  • Mats Jansson
  • Per Persson
  • Ulrik Istedt
  • Gerhard Groebner
  • Reiner Giesler
  • Juergen Schleucher
Publishing year: 2012
Language: English
Pages: 3950-3956
Publication/Series: Environmental Science & Technology
Volume: 46
Document type: Journal article
Publisher: The American Chemical Society (ACS)
Additional info: 7

Abstract english

Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.

Keywords

  • Earth and Related Environmental Sciences

Other

Published
  • ISSN: 1520-5851
Per Persson
E-mail: per [dot] persson [at] biol [dot] lu [dot] se

Professor

MEMEG

+46 46 222 17 96

+46 70 266 38 79

E-C350

50

Professor

Centre for Environmental and Climate Research (CEC)

+46 46 222 17 96

+46 70 266 38 79

D350

Ekologihuset, Sölvegatan 37, Lund

50