Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

A MYC2/MYC3/MYC4-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels

  • Alex van Moerkercke
  • Owen Duncan
  • Mark Zander
  • Jan Simura
  • Martyna Broda
  • Robin Vanden Bossche
  • Mathew G. Lewsey
  • Sbatie Lama
  • Karam B. Singh
  • Karin Ljung
  • Joseph R. Ecker
  • Alain Goossens
  • A. Harvey Millar
  • Olivier van Aken
Publishing year: 2019
Language: English
Pages: 23345-23356
Publication/Series: Proceedings of the National Academy of Sciences of the United States of America
Volume: 116
Issue: 46
Document type: Journal article
Publisher: National Acad Sciences

Abstract english

Mechanical stimuli, such as wind, rain, and touch affect plant development, growth, pest resistance, and ultimately reproductive success. Using water spray to simulate rain, we demonstrate that jasmonic acid (JA) signaling plays a key role in early gene-expression changes, well before it leads to developmental changes in flowering and plant architecture. The JA-activated transcription factors MYC2/MYC3/MYC4 modulate transiently induced expression of 266 genes, most of which peak within 30 min, and control 52% of genes induced >100-fold. Chromatin immunoprecipitation-sequencing analysis indicates that MYC2 dynamically binds >1,300 promoters and trans-activation assays show that MYC2 activates these promoters. By mining our multiomic datasets, we identified a core MYC2/MYC3/MYC4-dependent “regulon” of 82 genes containing many previously unknown MYC2 targets, including transcription factors bHLH19 and ERF109. bHLH19 can in turn directly activate the ORA47 promoter, indicating that MYC2/MYC3/MYC4 initiate a hierarchical network of downstream transcription factors. Finally, we also reveal that rapid water spray-induced accumulation of JA and JA-isoleucine is directly controlled by MYC2/ MYC3/MYC4 through a positive amplification loop that regulates JA-biosynthesis genes.


  • Botany
  • Genetics
  • Jasmonic acid
  • Mechanical stimulation
  • Plants
  • Touch
  • Transcription


  • ISSN: 0027-8424
Olivier van Aken
E-mail: olivier [dot] van_aken [at] biol [dot] lu [dot] se

Senior lecturer

Molecular Cell Biology

+46 46 222 94 13



Research group

Plant Biology



Doctoral students and postdocs

PhD students, main supervisor