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Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

Author:
  • Olivier Van Aken
  • Tamara Pecenková
  • Brigitte van de Cotte
  • Riet Maria De Rycke
  • Dominique Eeckhout
  • Hillel Fromm
  • Geert De Jaeger
  • Erwin Witters
  • Gerrit T S Beemster
  • Dirk Inzé
  • Frank Van Breusegem
Publishing year: 2007-12
Language: English
Pages: 64-850
Publication/Series: Plant Journal
Volume: 52
Issue: 5
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

The Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type-I (AtPHB3 and AtPHB4) and type-II (AtPHB1, AtPHB2 and AtPHB6) classes, based on their phylogenetic relationships with yeast PHB1 and PHB2, respectively. Yeast and animal PHBs are reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging and apoptosis. All transcribed Arabidopsis PHB genes are primarily expressed in both shoot and root proliferative tissues, where they are present in mitochondrial multimeric complexes. Loss of function of the type-I AtPHB4 had no phenotypic effects, while loss of function of the homologous AtPHB3 caused mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Double knockout atphb3 atphb4 plants were not viable, but transgenic lines overexpressing AtPHB3 or AtPHB4 showed leaf shape aberrations and an increased shoot branching phenotype. Genome-wide microarray analysis revealed that both knockout and overexpression perturbations of AtPHB3 and AtPHB4 provoked an altered abundance of mitochondrial and stress-related transcripts. We propose that plant type-I PHBs take part in protein complexes that are necessary for proficient mitochondrial function or biogenesis, thereby supporting cell division and differentiation in apical tissues.

Keywords

  • Biochemistry and Molecular Biology
  • Botany
  • Arabidopsis
  • Arabidopsis Proteins
  • Cell Division
  • Cluster Analysis
  • Meristem
  • Mitochondrial Proteins
  • Phenotype
  • Phylogeny
  • Protein Transport
  • Repressor Proteins

Other

Published
  • ISSN: 0960-7412
Olivier van Aken
E-mail: olivier [dot] van_aken [at] biol [dot] lu [dot] se

Senior lecturer

Molecular Cell Biology

+46 46 222 94 13

B-A330

4

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Plant Biology

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