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Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease

Author:
  • Ursula R Kees
  • Jette Ford
  • Marcia Watson
  • Ashleigh Murch
  • Markus Ringnér
  • Robert L Walker
  • Paul Meltzer
Publishing year: 2003
Language: English
Pages: 671-677
Publication/Series: Molecular Cancer Therapeutics
Volume: 2
Issue: 7
Document type: Journal article
Publisher: American Association for Cancer Research

Abstract english

The development of new drugs against cancer requires established cell lines. They are needed for in vitro studies to identify candidate drugs and in xenograft models to measure drug efficacy in vivo. Specific criteria need to be fulfilled by cell lines used in the evaluation of potential novel therapeutic agents. It is imperative that they display the features of the particular cancer under investigation. Given the documented heterogeneity of cancers, relevant subtypes need to be represented. In this study, we have examined these aspects for pediatric acute lymphoblastic leukemia. A panel of 13 leukemia cell lines recently established in our laboratory was analyzed. We used cDNA microarrays to define the gene expression profiles and compared the data with immunophenotyping and cytogenetic analyses. The expression profiles obtained showed excellent concordance with corresponding protein levels. Importantly, the panel of lines displayed the critical genetic features identified in clinically important acute lymphoblastic leukemia subtypes in childhood leukemia patients.

Keywords

  • Cancer and Oncology

Other

Published
  • ISSN: 1538-8514
Markus Ringnér
E-mail: markus [dot] ringner [at] biol [dot] lu [dot] se

Research engineer

Molecular Cell Biology

B-A317

Sölvegatan 35, Lund

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