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Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster

Author:
  • Mads Fristrup Schou
Publishing year: 2013
Language: English
Pages: 44-46
Publication/Series: Fly
Volume: 7
Issue: 1
Document type: Journal article
Publisher: Landes Bioscience

Abstract english

When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila.

Keywords

  • Ecology
  • Genetics
  • Egg-to-adult viability
  • density control
  • random sampling
  • reliability
  • sampling error

Other

Published
  • ISSN: 1933-6942
Mads Schou
E-mail: mads [dot] schou [at] biol [dot] lu [dot] se

Postdoctoral fellow

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