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Teaching about photosynthesis with simple equipment: analysis of light-induced changes in fluorescence and reflectance of plant leaves

  • Lars Olof Björn
  • Shaoshan Li
Publishing year: 2013
Language: English
Pages: 349-353
Publication/Series: Photosynthesis Research
Volume: 116
Issue: 2-3
Document type: Journal article
Publisher: Springer

Abstract english

Solar energy absorbed by plants results in either reflection or absorption. The latter results in photosynthesis, fluorescence, or heat. Measurements of fluorescence changes have been used for monitoring processes associated with photosynthesis. A simple method to follow

changes in leaf fluorescence and leaf reflectance associated with nonphotochemical quenching and light acclimation of leaves is described. The main equipment needed consists of a green-light emitting laser pointer, a digital camera, and a personal computer equipped with the camera acquisition software and the programs ImageJ and Excel. Otherwise,

only commonly available cheap materials are required.


  • Biological Sciences
  • Fluorescence
  • Acclimation
  • Reflectance
  • Spectrum
  • Xanthophyll cycle
  • Teaching


  • ISSN: 0166-8595
Lars Olof Björn
E-mail: lars_olof [dot] bjorn [at] biol [dot] lu [dot] se

Professor emeritus

Molecular Cell Biology

+46 46 222 72 53