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An artifact in measurements of in vivo light-induced absorbance changes

  • K. C. Hsiao
  • Lars Olof Björn
Publishing year: 1983
Language: English
Pages: 271-274
Publication/Series: Journal of Biochemical and Biophysical Methods
Volume: 8
Issue: 4
Document type: Journal article
Publisher: Elsevier

Abstract english

The effects of scattered actinic radiation on photomultipliers (Hamamatsu R-562) were investigated. Using cotton-wool to model dense biological preparations, it was found that the scattered actinic radiation received by the photomultiplier gives rise to phytochrome-like signals. This demonstrated the necessity to shield the photomultiplier from scattered actinic light for sensitive measurements with light-scattering preparations.


  • Biological Sciences


  • ISSN: 0165-022X
Lars Olof Björn
E-mail: lars_olof [dot] bjorn [at] biol [dot] lu [dot] se

Professor emeritus

Molecular Cell Biology

+46 46 222 72 53