Proteomic analysis of Allium cepa var. agrogarum L. roots under copper stress
- Molecular Cell Biology
Methods: Protein extraction; Two-dimensional electrophoresis (2-DE) analysis; Mass spectrometry analysis;
Establishment of the in-house database; Restriction enzyme map of the in-house database and protein
Results Root growth was dramatically inhibited after 12 h Cu treatment. By establishing an in-house database and using mass spectrometry analysis, 27 differentially abundant proteins were identified. These 27 proteins were involved in multiple biological processes including defensive response, transcription regulation and protein synthesis, cell wall synthesis, cell cycle and DNA replication, and other important functions.
Conclusions: Our results provide new insights at the proteomic level into the Cu-induced responses, defensive responses and toxic effects, and provide new molecular markers of the early events of plant responses to Cu toxicity. Moreover, the establishment of an in-house database provides a big improvement for proteomics
research on non-model plants.
- Biological Sciences
- Allium cepa var. agrogarum L .
- Defensive responses
- Toxic effects
- ISSN: 0032-079X