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Light as a tool for biologists: Recent developments

  • Lars Olof Björn
  • Lars Olof Björn
Publishing year: 2008
Language: English
Pages: 93-122
Document type: Book chapter
Publisher: Springer

Abstract english

After a brief introduction (1) this chapter covers: (2) optical tweezers and related techniques, (3) uses of lasers for ablation, desorption, ionization, and dissection, (4) fluorescent labeling, (5) Abbe's diffraction limit to spatial resolution in microscopy, (6) two-photon excitation fluorescence microscopy, (7) stimulated emission depletion (STED), (8) near-field microscopy, (9) quantum dots and their uses in biology, (10) photochemical internalization and caged compounds, (11) photogating of membrane channels, and (12) photo-crosslinking and photolabeling.


  • Biological Sciences
  • Optical tweezers Laser Ablation Desorption Ionization Dissection Fluorescent labeling Abbe's diffraction limit Spatial resolution Microscopy Two-photon excitation Stimulated emission depletion STEM Near-field microscopy Quantum dot Photochemical internalization Photogating Membrane channel Photocrosslinking Photolabeling DNA sequencing


  • Photobiology
  • Photobiology-lup-obsolete
  • ISBN: 978-0-387-72654-0
Lars Olof Björn
E-mail: lars_olof [dot] bjorn [at] biol [dot] lu [dot] se

Professor emeritus

Molecular Cell Biology

+46 46 222 72 53