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Agricultural land use determines functional genetic diversity of soil microbial communities

Author:
  • Lokeshwaran Manoharan
  • Sandeep K. Kushwaha
  • Dag Ahrén
  • Katarina Hedlund
Publishing year: 2017-12-01
Language: English
Pages: 423-432
Publication/Series: Soil Biology and Biochemistry
Volume: 115
Document type: Journal article
Publisher: Elsevier

Abstract english

Microbial communities play a major role in the degradation of soil organic matter (SOM) in soils. Despite its significance, the functional diversity of the highly diverse microbial communities is poorly understood. To address this, we applied a recently developed technique, captured metagenomics, to determine the effects of land-use on the functional genetic diversity of genes involved in the carbon degradation of SOM in five pairs of agricultural soils with either winter wheat or grass as management. In addition, 16S rRNA based amplicon sequencing was used to study the taxonomic composition in the same soils. The functional genes resulting from the captured metagenomes had a higher abundance and diversity of sequences coding for enzymes degrading SOM in the grasslands compared to the wheat soils. Though the taxonomic diversity did not correlate with the land use. Amounts of C and N (organic matter content) in the soils affected both functional and taxonomic diversity of the microbial communities, where N was highly correlated to their functions and C was highly correlated to their taxonomy. Captured metagenomic analyses of the functional genes may provide a measure of the potential SOM degradation capacity by soil microbial communities at a high resolution. This can be used for assessments of how agricultural management affects the functioning of soil communities.

Keywords

  • Microbiology
  • Environmental Sciences related to Agriculture and Land-use
  • Captured metagenomics
  • Functional genes
  • Land-use management
  • Microbial ecology
  • MiSeq paired-end sequencing

Other

Published
  • ISSN: 0038-0717