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Acoustic trapping efficiency of nanoparticles and bacteria

Author:
  • Mikael Evander
  • Björn Hammarström
  • Pelle Ohlsson
  • Thomas Laurell
  • Johan Nilsson
Publishing year: 2012
Language: English
Pages: 515-517
Publication/Series: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Document type: Conference paper
Publisher: Chemical and Biological Microsystems Society

Abstract english

In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow focusing ratio of 1:10, GFP-producing E. coli could be trapped at an efficiency of above 95%. Using this characterization technique, important aspects of the acoustic trapping method (e.g. transducer frequency and voltage, size and type of seeding particle, amount of flow focusing, total flow rate etc.) can be characterized and optimized.

Keywords

  • Biomedical Laboratory Science/Technology
  • Acoustic trapping
  • Bacteria
  • Nanoparticles
  • Trapping efficiency

Other

16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
2012-10-28 - 2012-11-01
Okinawa, Japan
Published
  • ISBN: 9780979806452