Portrait of Jadranka Rota. Photo.

Jadranka Rota

Museum curator

Portrait of Jadranka Rota. Photo.

PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

Author

  • Niklas Wahlberg
  • Carlos Peña
  • Milla Ahola
  • Christopher W. Wheat
  • Jadranka Rota

Summary, in English

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Topic

  • Zoology
  • Biochemistry and Molecular Biology
  • Genetics

Keywords

  • Lepidoptera
  • Molecular systematics
  • Phylogenetics
  • Phylogenomics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1313-2989