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Equipment

Among other things we have the equipment below at the department. Before you buy new equipment check here and see if you may use any of the existing machinery instead.

3D printer Makerbot Replicator 2

Use: A 3D printer for making three-dimensional objects in plastic is available in the Animal Physiology lab. Can only be used upon agreement. Knowledge in CAD-programs, like TinkerCAD, is needed.

Location: Biology Building C, ground floor, Animal Physiology.

Price: On agreement.

Contact person: Per_Fredrik [dot] Johansson [at] biol [dot] lu [dot] se, +46 46 222 93 51, Animal Physiology.


Flow cam – Imaging Particle Analysis System

Use: A FlowCAM (Fluid Imaging Techn.) is located in the Ecology Building. It is a hight throughput particle analysis system for microscopic particles (e. g. cyanobacteria, fossil cysts, nematodes, malaria parasites, red blood cells, phytoplankton, zooplankton) that provides the user with a number of variables (e. g. volume, fluorescence) and images of each particle. The system includes software to process data.

Location: Ecology Building, Aquatic Ecology unit.

Price: On agreement.

Contact person: Marie [dot] Svensson [at] biol [dot] lu [dot] se, +46 46 222 37 51, Aquatic Ecology.


Flow cytometer (FCM) Accurri BD

Use:A flow cytometer, BD Accuri C6 (Oskar) is located in Biology Building C. It can be used to measure various physical and chemical properties of cells and particles with high precision. It is used both in reseach and in teaching. Examples of applications are determination of cell surface and intracellular proteins, enzyme activities, apoptosis, cell cycle phase distribution, cell and mitochondrial membrane potentials. The FCM was purchased 2012.

Location: Biology Building C, first floor, Animal Physiology.

Price: On agreement.

Contact person: Stina [dot] Oredsson [at] biol [dot] lu [dot] se, +46 46 222 94 97, Animal Physiology.


Flow cytometer

The other BD Accuri C6 is placed in the biosafety level 2 lab in Biology House A and used for pathogenic microorganisms. This machine is also equipped with a selectable laser module for cytometric bead array analysis and a CSampler Plus robotic arm that enables high throughput analyses using 96-well plates.

Contact person: Fredric [dot] Carlsson [at] biol [dot] lu [dot] se, +46 46 222 4081, Molecular Cell Biology.


GC-FID for headspace samples

Use: This gas chromatograph can be used for measuring soil respiration (carbon dioxide), methane production, N2-fixation activity (acetylene reduction), ethylene etc in headspace samples. The instrument is an YL Instrument 6500 GC with a HTA autosampler for 40 samples, purchased 2014. The machine can only be used upon agreement with the contact person.

Location: Ecology Building, 3rd floor, Biodiversity unit.

Cost: Annual user fee plus charged at cost-price per sample. With owned sampling equipment (vials, caps, etc.) running costs are presently less than 10 SEK/sample.

Contact: Johannes [dot] Rousk [at] biol [dot] lu [dot] se, +46 46 222 37 45, MEMEG.


GC-MS lab, Pheromone Group

Use: Our GC lab is equipped with two GC-MS (gas chromatography with mass selective detector) and GC (gas chromatograph). These instruments can be used for the detection and identification of trace amounts down to picogram level of most organic volatiles, such as plant substances, incorporated stable isotopes or metabolites of pheromone intermediates.

Equipment:

  • GC Agilent 7890A with masspectrometer 5975C with inert Triple-axis EI. It is equipped with an Automatic Liquid Sampler (ALS) for 150 samples. Purchased 2013.
  • GC Agilent 6890 with masspectrometer 5973 EI and ALS for 100 samples.
  • GC Hewlett-Packard 5890 Plus with FID (Flame Ionizing Detector) to test sample quality, purity or quantification. Very high sensitivity.

All GC:s can be equipped with different types of columns; non-polar, polar or chiral special columns to analyze different types of samples. The software is ChemStation in all instruments. The mass spectrometers have several different MS library, e.g. latest NIST.

Sample preparation can be done in the lab. We also have equipment for odour collections with mini-pumps and filters in the lab or in the field (Porapac, Tenax, active carbon), SPME and a "closed-loop pump".

Location: Ecology Building, First floor, Pheromone group.

Price: 60 SEK/sample (+ trigger)

Contact persons: Hong-Lei [dot] Wang [at] biol [dot] lu [dot] se, +46 46 222 01 26 and Erling [dot] Jirle [at] biol [dot] lu [dot] se, +46 46 222 49 99, Pheromone Group.


HPLC

Use: By using HPLC (High Pressure Liquid Chromatography) you can separate and purify complex mixtures of enzymes/proteins/macromolecules in the µg/ng levels. Fractions can be collected for the analysis of e.g. enzyme activities, isotopes (15-N and 13-C), spectroscopy, and mass spectrometry.

A large number of columns are available (ion exchange, size exlusion, hydrophobic interaction, reversed phase). The machine is a Thermo Scientific Ultimate 3000 purchased 2012.

Use: Analyses of dissolved organic matter, extracellular enzymes and proteins in soils.

Location: Ecology Building, First floor, MEMEG.

Price: By agreement.

Contact person: Anders [dot] Tunlid [at] biol [dot] lu [dot] se, +46 46 222 37 52, MEMEG.


Integrated vibrational spectroscopy

Use: The "Integrated vibrational spectroscopy – microtom laboratory for molecular scale biogeochemial research" is located in the Ecology Building. It has the capability to record infrared and Raman spectra of essentially any sample in solid or liquid state. Infrared spectra can be collected at high time-resolution in transmission and reflection mode, and a special accessory designed and built by our group facilitates analysis of processes at solid-liquid interfaces in real-time and variable solution conditions. Moreover, the laboratory is equipped with FTIR and dispersive Raman microscopes that enables collection of spatially resolved infrared and Raman spectra of resolution of c. 5 and 0.5 µm, respectively.

Location: Ecology Building, Microbial Ecology, MEMEG unit.

Price: Access mainly through collaborative research projects. Experienced users can receive instrument time, if available, for a minor cost.

Contact person: Per Persson, Per [dot] Persso [at] biol [dot] lu [dot] se, +46 46 222 1796, MEMEG/CEC.


Liquid scintillator Perkin Elmer TriCarb

Use: To trace radioactive isotopes (alpha, beta, gamma) for measuring bacterial growth (3H-Leu incorporation), fungal growth (14C acetate incorporation), 14CO2 traps etc. The scintillator was purchased 2014.

Location: Ecology Building, second floor

Price: Annual user fee plus charged at cost-price per sample. With owned sampling equipment (vials, scintillation cocktail etc.) running costs are presently less than 10 SEK/sample.

Contact: Johannes [dot] Rousk [at] biol [dot] lu [dot] se, +46 46 222 37 45, MEMEG.


Microplate reader FLUOstar Omega

Use: The microplate reader has luminiscense and polarized fluorscense. It can be used for all sorts of cellular, biochemical, molecular and epigenetical assays.

Applications includes for example:

  • Monitoring absorbance changes in cellular co-factors NADH and NADPH allowing the determination of enzymatic activity
  • ELISA, DNA and Bradford protein quantitation assays
  • Reaction optimization (metallation of porphyrin in twelve different solvents) by parallel kinetic studies
  • Aggregation assays with e.g. Thioflavin T
  • Evaluation of PARP inhibitors
  • Measuring mitochondrial membrane potential
  • Reporter gene assays: GFP, YFP, luciferase, dual luciferase, BRET
  • ORAC, Determination of antioxidant activity, oxygen consumption and ROS determination
  • Protein, DNA and RNA quantification (e.g. Bradford, Absorbance 260/280, Quant-iT PicoGreen)

Location: Ecology Building, second floor

User rules: First time users get a short introduction. Booking calendar is placed by the machine. For long runs contact Caroline Isaksson well in advance.

Price: Not set yet.

Contact: Caroline [dot] Isaksson [at] biol [dot] lu [dot] se, +46 46 222 17 80, Evolutionary Ecology.


Microplate reader

Use: In Biology Building C there is one more plate reader, installed 2017, a SpectraMax i3 Multi-mode microplate platform (Molecular Devices), with a SpectraMax MiniMax Imaging Cytometer. It measures spectral-based absorbance, fluorescence, and luminescence. It also has modular upgrades for Western Blot, imaging, and fast kinetics with injectors.

Location: Biology Building C, Animal Physiology unit.

Price: Not set yet.

Contact person:Lars [dot] Raberg [at] biol [dot] lu [dot] se, +46 46 222 3766, Animal Physiology Group, Functional Zoology.


Microscope system for live single-cell imaging and bacterial cell biology

Use: This setup is placed in Biology Building A and is used for bacterial cell biology. It consists of the following: A microfluid cell perfusion system (CellASIC ONIX Microfluid platform), an inverted fluorescence microscope (Zeiss Axio Observer Z1) plus one older microscope as well, two sCMOS microscope cameras (Hamamatsu ORCA-Flash 4.0), software (Velocity 3D Image Analysis) and a micropoint laser system for photactivation and photobleaching.

Location: Biology Building A, Molecular Cell Biology Unit.

Contact person: Klas Flärdh, Klas [dot] Flardh [at] biol [dot] lu [dot] se, +46 46 222 8584, Molecular Cell Biology.


Molecular Imager

Use: A Typhoon FLA9500 Molecular imager (GE Healthcare) is located in the Biology Building A. This is a multi-imaging system for fluorescence and radioisotope detection and quantitative analysis of biomolecules. It detects radioactive signals from gels or membranes by phosphor image screens. Gels and membranes can be scanned for fluorescence signals.

Location: Biology Building A, Molecular Cell Biology Unit.

Price: Not set. This is not an infrastructure providing a service platform. However, the instrument has the unique property of radiation detection, which is very helpful for various molecular approaches, and such an apparatus is not very abundant.

Contact person: Marita [dot] Cohn [at] biol [dot] lu [dot] se, +46 46 222 7256, Molecular Cell Biology.


Picarro Cavity ring-down spectrometer

Use: A Picarro G2201-i dual carbon isotope analyzer is located in the Ecology Building. Cavity ring-down spectrometry represents a new technique to measure the concentration of dissolved inorganic carbon, CO2 and CH4, which enable researchers to detect variations in e.g. soil respiration rates at the scale of minutes. It can also generate long time-series of high resolution data in situ as well as in laboratory based microcosm experiments. An additional important benefit is that it can measure the isotopic composition of CO2 and CH4 with high precision.

Location: Ecology Building, Biodiversity unit, GC-lab.

Price: On agreement.

Contact person: Per [dot] Bengtson [at] biol [dot] lu [dot] se, +46 46 222 3760, Microbial Ecology Group, MEMEG.


Real-time PCR instrument (qPCR)

Use: An Agilent Stratagene Mx3005P qPCR instrument is located in the Ecology Building. Real-time polymerase chain reaction monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. The instrument can measure relative and absolute quantities of specific DNA or RNA molecules, e.g. infection intensities of various pathogens (DNA) or expression profiles of genes (mRNA).

Location: Ecology Building, MEMEG unit.

Price: The instrument is open for use by any researcher affiliated to the Department of Biology. There is no user fee though reagents and consumables (plastics) payed by the user.

Contact person: Staffan [dot] Bensch [at] biol [dot] lu [dot] se, +46 46 222 4292, MEEL, MEMEG.


Ultra centrifuges

A set of ultra centrifuges are available in Biology House A: Beckman L7-65, Beckman L8, Beckman-Coultier Avanti J-26 XP (4 of them) and more. These are for internal use within House A, but can also be used by others after agreement.

Location: Biology Building A, ground floor, Molecular Cell Biology.

Price: On agreement.

Contact persons: Elisabeth [dot] Barane [at] biol [dot] lu [dot] se, +46 46 222 77 94 or Lars [dot] Hederstedt [at] biol [dot] lu [dot] se, +46 46 222 86 22, Molecular Cell Biology.

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VISITING ADDRESSES

Biologihuset
Sölvegatan 35
223 62 Lund
Service point 4

Ecology Building
Sölvegatan 37
223 62 Lund
Service point 50

Arkivcentrum Syd
Porfyrvägen 20
224 78 Lund
Service point 62

DELIVERY ADDRESSES

Biology Building
general deliveries

Sölvegatan 35B
223 62 Lund

Biology Building
House D

Naturvetarvägen 2
223 62 Lund

Ecology Building
Naturvetarvägen 6A
223 62 Lund

Arkivcentrum Syd
Porfyrvägen 20
224 78 Lund

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