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Phylogeny of Xanthoria calcicola and X. parietina, based on rDNA ITS sequences.

Author:
  • Niklas Franc
  • Ingvar Kärnefelt
Publishing year: 1998
Language: English
Pages: 49-54
Publication/Series: Graphis Scripta
Volume: 9
Issue: 2
Document type: Journal article
Publisher: Nordisk lichenologisk förening, NLF

Abstract english

The relationship between the two lichen species Xanthoria calcicola and X.

parietina was investigated at genetic level, comparing the ITS regions of the

rDNA. The extracted DNA was amplified with the primers ITS4 and ITS5 in

a PCR and automatically sequenced. The variation within the separate

species was smaller than the variation between the species. Compared to a

third species, X. elegans, the variation was smaller between X. parietina and

X. calcicola than between any of them compared to X. elegans. When the

sequences was evaluated in the phylogenetic computer software Phylo_win

with eleven other lichen species, the genus Xanthoria was clearly separated

and the investigated species tn Xanthoria showed the same differentiated

pattern as above. These results clearly demonstrate that X. calcicola and X.

parietina are two closely related but genetically distinct species.

Keywords

  • Botany

Other

Published
  • ISSN: 0901-7593
Ingvar Kärnefelt
E-mail: ingvar [dot] karnefelt [at] biol [dot] lu [dot] se

Professor emeritus

Botanical collections

+46 46 222 89 78

+46 70 918 01 79

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