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Anthropogenic nitrogen enrichment enhances soil carbon accumulation by impacting saprotrophs rather than ectomycorrhizal fungal activity

  • Nadia I. Maaroufi
  • Annika Nordin
  • Kristin Palmqvist
  • Niles J. Hasselquist
  • Benjamin Forsmark
  • Nicholas P. Rosenstock
  • Håkan Wallander
  • Michael J. Gundale
Publishing year: 2019-06-05
Language: English
Pages: 2900-2914
Publication/Series: Global Change Biology
Volume: 25
Issue: 9
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

There is evidence that anthropogenic nitrogen (N) deposition enhances carbon (C) sequestration in boreal forest soils. However, it is unclear how free-living saprotrophs (bacteria and fungi, SAP) and ectomycorrhizal (EM) fungi responses to N addition impact soil C dynamics. Our aim was to investigate how SAP and EM communities are impacted by N enrichment and to estimate whether these changes influence decay of litter and humus. We conducted a long-term experiment in northern Sweden, maintained since 2004, consisting of ambient, low N additions (0, 3, 6, and 12 kg N ha−1 year−1) simulating current N deposition rates in the boreal region, as well as a high N addition (50 kg N ha−1 year−1). Our data showed that long-term N enrichment impeded mass loss of litter, but not of humus, and only in response to the highest N addition treatment. Furthermore, our data showed that EM fungi reduced the mass of N and P in both substrates during the incubation period compared to when only SAP organisms were present. Low N additions had no effect on microbial community structure, while the high N addition decreased fungal and bacterial biomasses and altered EM fungi and SAP community composition. Actinomycetes were the only bacterial SAP to show increased biomass in response to the highest N addition. These results provide a mechanistic understanding of how anthropogenic N enrichment can influence soil C accumulation rates and suggest that current N deposition rates in the boreal region (≤12 kg N ha−1 year−1) are likely to have a minor impact on the soil microbial community and the decomposition of humus and litter.


  • Soil Science
  • carbon sequestration
  • ecological stoichiometry
  • Gadgil effect
  • high-throughput sequencing
  • ingrowth mesh bags
  • ITS amplicons
  • litter decomposition
  • root exclosure
  • soil organic matter


  • ISSN: 1354-1013
Håkan Wallander
E-mail: hakan [dot] wallander [at] biol [dot] lu [dot] se



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