Microfungi were isolated from unpolluted and copper polluted forest soils and their tolerance was tested on agar plates. Species from polluted sites were usually tolerant to Cu. The species were Paecilomyces farinosus, Beauveria bassiana, Verticillium spp. and Penicillium pactum. Sensitive species were found among Mortierella, Oidiodendron and Penicillium. usually rare in polluted sites. Little evidence for adaptation to Cu was found among isolates with short or long history of pollution.