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Application of antisense transformation of a barley chitinase in studies of arbuscule formation by a mycorrhizal fungus

Author:
  • U Troedsson
  • Pål Axel Olsson
  • Carin Jarl-Sunesson
Publishing year: 2005
Language: English
Pages: 65-72
Publication/Series: Hereditas
Volume: 142
Issue: 1
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

Barley (Hordeum vulgare L.) plants of two commercial cultivars were transformed with sense and antisense constructs of a chitinase class II gene in order to develop a transformation system for this gene in barley. Transformation of embryos with the two antisense constructs resulted in ten regenerated plants, while no plants could be obtained using the sense construct. The presence of the inserted construct could be confirmed for six of the plants by PCR analysis. This system was used to study the role of class II chitinase in the regulation of mycorrhizal symbiosis. The colonization of two of the antisense transformants by the arbuscular mycorrhizal fungus Glomus intraradices was investigated microscopically and by use of signature fatty acids. The arbuscular incidence increased in transformed barley, and one transformant supported higher extraradical mycelium biomass. It is concluded that antisense transformation of barley could be a useful tool in investigations on the symbiosis between barley and mycorrhizal fungi.

Keywords

  • Genetics

Other

Published
  • Microbial Ecology
  • ISSN: 1601-5223
Carin Jarl-Sunesson
E-mail: carin [dot] jarl-sunesson [at] biol [dot] lu [dot] se

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Department of Biology

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Molecular Cell Biology

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Research group

Plant Biology

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PhD students & postdocs

Assistant supervisor

Yihan Xia