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A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

Author:
  • Inaki Iturbe-Ormaetxe
  • Megan Woolfit
  • Edwige Rances
  • Anne Duplouy
  • Scott L. O'NEill
Publishing year: 2011
Language: English
Pages: 134-136
Publication/Series: Journal of Microbiological Methods
Volume: 84
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.

Keywords

  • Genetics

Other

Published
  • ISSN: 1872-8359
Anne Duplouy
E-mail: anne [dot] duplouy [at] biol [dot] lu [dot] se

Researcher

Biodiversity

Sölvegatan 37, Lund

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