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A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.

Author:
  • Anna Rasmussen
  • Y Lv
  • Klaus Schnackerz
  • Jure Piskur
Publishing year: 2011
Language: English
Pages: 1227-1232
Publication/Series: Nucleosides, Nucleotides & Nucleic Acids
Volume: 30
Issue: 12
Document type: Journal article
Publisher: Taylor & Francis

Abstract english

We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.

Keywords

  • Biological Sciences
  • Expression vector
  • Saccharomyces (Lachancea) kluyveri
  • URC pathway
  • catabolism of pyrimidines

Other

Published
  • ISSN: 1525-7770
Anna Andersson Rasmussen
E-mail: anna [dot] rasmussen [at] biol [dot] lu [dot] se

Research engineer

Molecular Cell Biology

+46 46 222 86 31

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