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Transformation of the nematode-trapping fungus Arthrobotrys oligospora

Author:
  • A. Tunlid
  • J. Åhman
  • R. P. Oliver
Publishing year: 1999-04-01
Language: English
Pages: 111-116
Publication/Series: FEMS Microbiology Letters
Volume: 173
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.

Keywords

  • Microbiology
  • β-Glucuronidase reporter gene
  • Hygromycin resistance
  • Nematode-trapping fungi
  • Restriction mediated integration
  • Transformation
  • Biologi

Other

Published
  • ISSN: 0378-1097
Anders Tunlid
E-mail: anders [dot] tunlid [at] biol [dot] lu [dot] se

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