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Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

Author:
  • G. Odham
  • A. Tunlid
  • L. Larsson
  • P. A. Mårdh
Publishing year: 1982-12-01
Language: English
Pages: 83-86
Publication/Series: Chromatographia
Volume: 16
Issue: 1
Document type: Journal article
Publisher: Vieweg Verlag

Abstract english

Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.

Keywords

  • Analytical Chemistry
  • Chiral capillary GC
  • D- and L-alanine
  • Diaminopimelic acid
  • Fused silica capillary GC
  • Mass spectrometry
  • Muramic acid
  • Selected ion monitoring
  • Tuberculostearic acid

Other

Published
  • ISSN: 0009-5893
Anders Tunlid
E-mail: anders [dot] tunlid [at] biol [dot] lu [dot] se

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