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Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS

Author:
  • D Apoga
  • B Ek
  • Anders Tunlid
Publishing year: 2001
Language: English
Pages: 145-150
Publication/Series: FEMS Microbiology Letters
Volume: 197
Issue: 2
Document type: Journal article
Publisher: Elsevier

Abstract english

A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights, reserved.

Keywords

  • Biological Sciences

Other

Published
  • Microbial Ecology
  • ISSN: 1574-6968
Anders Tunlid
E-mail: anders [dot] tunlid [at] biol [dot] lu [dot] se

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