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Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.

Author:
  • Jana Braesel
  • Sebastian Götze
  • Firoz Shah
  • Daniel Heine
  • James Tauber
  • Christian Hertweck
  • Anders Tunlid
  • Pierre Stallforth
  • Dirk Hoffmeister
Publishing year: 2015
Language: English
Pages: 1325-1334
Publication/Series: Chemistry and Biology
Volume: 22
Issue: 10
Document type: Journal article
Publisher: Cell Press

Abstract english

The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.

Keywords

  • Microbiology
  • Forest Science

Other

Published
  • MICCS - Molecular Interactions Controlling soil Carbon Sequestration
  • Microbial Ecology
  • ISSN: 1879-1301
Anders Tunlid
E-mail: anders [dot] tunlid [at] biol [dot] lu [dot] se

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