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Cold stress decreases the capacity for respiratory NADH oxidation in potato leaves.

Author:
  • Staffan Svensson
  • Fredrik I Johansson
  • Ian M Møller
  • Allan Rasmusson
Publishing year: 2002
Language: English
Pages: 79-82
Publication/Series: FEBS Letters
Volume: 517
Issue: 1-3
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

Cold stress effects on the expression of genes for respiratory chain enzymes were investigated in potato (Solanum tuberosum L., cv. Desiree) leaves. The nda1 and ndb1 genes, homologues to genes encoding the non-proton-pumping respiratory chain NADH dehydrogenases of Escherichia coli and yeast, were compared to genes encoding catalytic subunits of the proton-pumping NADH dehydrogenase (complex I). Using a real-time PCR system, we demonstrate a specific and gradual decrease of the NDA1 transcript after exposing the plants to 5 degrees C. After 6 days of cold treatment the NDA1 transcript abundance is 10% of the original level. This decrease is accompanied by specific decreases of immunodetected NDA protein and internal rotenone-insensitive NADH oxidation in mitochondria isolated from cold-treated plants. The alternative oxidase is not cold-induced neither at the protein nor at the activity level. The results are discussed in relation to the recent finding that the nda1 gene expression is completely light-dependent.

Keywords

  • Biological Sciences
  • Plant
  • Rotenone : pharmacology
  • Cold
  • RNA
  • Messenger : biosynthesis
  • Mitochondria : enzymology
  • NADH Dehydrogenase : genetics
  • NADH Dehydrogenase : metabolism
  • Oxidation-Reduction : drug effects
  • Plant Leaves : enzymology
  • Oxidoreductases : metabolism
  • Plant Leaves : genetics
  • Potatoes : enzymology
  • Potatoes : genetics
  • Messenger : analysis
  • Genes
  • Gene Expression Regulation

Other

Published
  • ISSN: 1873-3468
Allan Rasmusson
E-mail: allan [dot] rasmusson [at] biol [dot] lu [dot] se

Professor

Molecular Cell Biology

+46 46 222 93 81

B-A329A

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