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Short-term ammonium supply induces cellular defence to prevent oxidative stress in Arabidopsis leaves

Author:
  • Anna Podgórska
  • Maria Burian
  • Anna M. Rychter
  • Allan G. Rasmusson
  • Bozena Szal
Publishing year: 2017-05
Language: English
Pages: 65-83
Publication/Series: Physiologia Plantarum
Volume: 160
Issue: 1
Document type: Journal article
Publisher: Wiley Online Library

Abstract english

Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and mitochondrial alternative electron transport pathways, indicating rapid but temporally separated changes in chloroplastic, mitochondrial and cytosolic ROS metabolism. The fast induction of antioxidant defences significantly lowered intracellular H2O2 levels, and thus protected Arabidopsis leaves from oxidative stress. On the other hand elevated extracellular ROS production in response to ammonium supply may be involved in signalling. The response pattern displays an intricate plasticity of Arabidopsis redox metabolism to minimise stress in responses to nutrient changes.

Keywords

  • Biochemistry and Molecular Biology
  • Botany

Other

Published
  • ISSN: 0031-9317
Allan Rasmusson
E-mail: allan [dot] rasmusson [at] biol [dot] lu [dot] se

Professor

Molecular Cell Biology

+46 46 222 93 81

B-A329A

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