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Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria

  • Allan G. Rasmusson
  • Kenneth M. Fredlund
  • Ian M. Møller
Publishing year: 1993-02-08
Language: English
Pages: 107-110
Publication/Series: Biochimica et Biophysica Acta - Bioenergetics
Volume: 1141
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide.


  • Biochemistry and Molecular Biology
  • (Plant)
  • Enzyme purification
  • Inner membrane
  • Mitochondrion
  • NAD(P)H dehydrogenase
  • Protein purification
  • Respiratory chain
  • Rotenone insensitivity


  • ISSN: 0005-2728
Allan Rasmusson
E-mail: allan [dot] rasmusson [at] biol [dot] lu [dot] se


Molecular Cell Biology

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