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Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Author:
  • Federico Gomez
  • Petr Dejmek
  • Krister Lundgren
  • Allan Rasmusson
  • Antonio Vicente
  • Thomas Moritz
Publishing year: 2009
Language: English
Pages: 469-479
Publication/Series: Planta
Volume: 230
Issue: 3
Document type: Journal article
Publisher: Springer

Abstract english

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

Keywords

  • Food Engineering
  • Biological Sciences
  • Electroporation
  • Cell membrane recovery
  • Metabolic analysis
  • multivariate data analysis
  • OPLS
  • Wounding
  • Stress response

Other

Published
  • ISSN: 0032-0935
Allan Rasmusson
E-mail: allan [dot] rasmusson [at] biol [dot] lu [dot] se

Professor

Molecular Cell Biology

+46 46 222 93 81

B-A329A

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