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An engineered combinatorial module of transcription factors boosts production of monoterpenoid indole alkaloids in Catharanthus roseus

Author:
  • Fabian Schweizer
  • Maite Colinas
  • Jacob Pollier
  • Alex Van Moerkercke
  • Robin Vanden Bossche
  • Rebecca de Clercq
  • Alain Goossens
Publishing year: 2018-07
Language: English
Pages: 150-162
Publication/Series: Metabolic Engineering
Volume: 48
Document type: Journal article
Publisher: Elsevier

Abstract english

To fend off microbial pathogens and herbivores, plants have evolved a wide range of defense strategies such as physical barriers, or the production of anti-digestive proteins or bioactive specialized metabolites. Accumulation of the latter compounds is often regulated by transcriptional activation of the biosynthesis pathway genes by the phytohormone jasmonate-isoleucine. Here, we used our recently developed flower petal transformation method in the medicinal plant Catharanthus roseus to shed light on the complex regulatory mechanisms steering the jasmonate-modulated biosynthesis of monoterpenoid indole alkaloids (MIAs), to which the anti-cancer compounds vinblastine and vincristine belong. By combinatorial overexpression of the transcriptional activators BIS1, ORCA3 and MYC2a, we provide an unprecedented insight into the modular transcriptional control of MIA biosynthesis. Furthermore, we show that the expression of an engineered de-repressed MYC2a triggers a tremendous reprogramming of the MIA pathway, finally leading to massively increased accumulation of at least 23 MIAs. The current study unveils an innovative approach for future metabolic engineering efforts for the production of valuable bioactive plant compounds in non-model plants.

Keywords

  • Ecology

Other

Published
  • ISSN: 1096-7176
Alex Van Moerkercke
E-mail: alex [dot] van_moerkercke [at] biol [dot] lu [dot] se

Postdoctoral fellow

Molecular Cell Biology

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Research group

Plant Biology

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Olivier Van Aken