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CCoAOMT Down-Regulation Activates Anthocyanin Biosynthesis in Petunia

Author:
  • Nur Fariza M Shaipulah
  • Joëlle K Muhlemann
  • Benjamin D Woodworth
  • Alex Van Moerkercke
  • Julian C Verdonk
  • Aldana A Ramirez
  • Michel A Haring
  • Natalia Dudareva
  • Robert C Schuurink
Publishing year: 2016-02
Language: English
Pages: 717-731
Publication/Series: Plant Physiology
Volume: 170
Issue: 2
Document type: Journal article
Publisher: American Society of Plant Biologists

Abstract english

Anthocyanins and volatile phenylpropenes (isoeugenol and eugenol) in petunia (Petunia hybrida) flowers have the precursor 4-coumaryl coenzyme A (CoA) in common. These phenolics are produced at different stages during flower development. Anthocyanins are synthesized during early stages of flower development and sequestered in vacuoles during the lifespan of the flowers. The production of isoeugenol and eugenol starts when flowers open and peaks after anthesis. To elucidate additional biochemical steps toward (iso)eugenol production, we cloned and characterized a caffeoyl-coenzyme A O-methyltransferase (PhCCoAOMT1) from the petals of the fragrant petunia 'Mitchell'. Recombinant PhCCoAOMT1 indeed catalyzed the methylation of caffeoyl-CoA to produce feruloyl CoA. Silencing of PhCCoAOMT1 resulted in a reduction of eugenol production but not of isoeugenol. Unexpectedly, the transgenic plants had purple-colored leaves and pink flowers, despite the fact that cv Mitchell lacks the functional R2R3-MYB master regulator ANTHOCYANIN2 and has normally white flowers. Our results indicate that down-regulation of PhCCoAOMT1 activated the anthocyanin pathway through the R2R3-MYBs PURPLE HAZE (PHZ) and DEEP PURPLE, with predominantly petunidin accumulating. Feeding cv Mitchell flowers with caffeic acid induced PHZ expression, suggesting that the metabolic perturbation of the phenylpropanoid pathway underlies the activation of the anthocyanin pathway. Our results demonstrate a role for PhCCoAOMT1 in phenylpropene production and reveal a link between PhCCoAOMT1 and anthocyanin production.

Keywords

  • Biochemistry and Molecular Biology
  • Acyl Coenzyme A
  • Anthocyanins
  • Down-Regulation
  • Eugenol
  • Flowers
  • Gene Expression Regulation, Plant
  • Methyltransferases
  • Petunia
  • Phenotype
  • Plant Leaves
  • Plant Proteins
  • Plants, Genetically Modified
  • Recombinant Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

Other

Published
  • ISSN: 1532-2548
Alex Van Moerkercke
E-mail: alex [dot] van_moerkercke [at] biol [dot] lu [dot] se

Postdoctoral fellow

Molecular Cell Biology

+46 46 222 49 49

B-A308

Sölvegatan 35, Lund

4

Research group

Plant Biology

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Postdoc host

Olivier Van Aken