Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

CathaCyc, a metabolic pathway database built from Catharanthus roseus RNA-Seq data

  • Alex Van Moerkercke
  • Michele Fabris
  • Jacob Pollier
  • Gino J E Baart
  • Stephane Rombauts
  • Ghulam Hasnain
  • Heiko Rischer
  • Johan Memelink
  • Kirsi-Marja Oksman-Caldentey
  • Alain Goossens
Publishing year: 2013-05
Language: English
Pages: 673-685
Publication/Series: Plant and Cell Physiology
Volume: 54
Issue: 5
Document type: Journal article
Publisher: Oxford University Press

Abstract english

The medicinal plant Madagascar periwinkle (Catharanthus roseus) synthesizes numerous terpenoid indole alkaloids (TIAs), such as the anticancer drugs vinblastine and vincristine. The TIA pathway operates in a complex metabolic network that steers plant growth and survival. Pathway databases and metabolic networks reconstructed from 'omics' sequence data can help to discover missing enzymes, study metabolic pathway evolution and, ultimately, engineer metabolic pathways. To date, such databases have mainly been built for model plant species with sequenced genomes. Although genome sequence data are not available for most medicinal plant species, next-generation sequencing is now extensively employed to create comprehensive medicinal plant transcriptome sequence resources. Here we report on the construction of CathaCyc, a detailed metabolic pathway database, from C. roseus RNA-Seq data sets. CathaCyc (version 1.0) contains 390 pathways with 1,347 assigned enzymes and spans primary and secondary metabolism. Curation of the pathways linked with the synthesis of TIAs and triterpenoids, their primary metabolic precursors, and their elicitors, the jasmonate hormones, demonstrated that RNA-Seq resources are suitable for the construction of pathway databases. CathaCyc is accessible online ( and offers a range of tools for the visualization and analysis of metabolic networks and 'omics' data. Overlay with expression data from publicly available RNA-Seq resources demonstrated that two well-characterized C. roseus terpenoid pathways, those of TIAs and triterpenoids, are subject to distinct regulation by both developmental and environmental cues. We anticipate that databases such as CathaCyc will become key to the study and exploitation of the metabolism of medicinal plants.


  • Biochemistry and Molecular Biology
  • Catharanthus
  • Cluster Analysis
  • Cyclopentanes
  • Databases as Topic
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Metabolic Networks and Pathways
  • Molecular Sequence Annotation
  • Oxylipins
  • RNA, Plant
  • Secologanin Tryptamine Alkaloids
  • Sequence Analysis, RNA
  • Transcriptome
  • Journal Article
  • Research Support, Non-U.S. Gov't


  • ISSN: 1471-9053
Alex Van Moerkercke
E-mail: alex [dot] van_moerkercke [at] biol [dot] lu [dot] se

Postdoctoral fellow

Molecular Cell Biology

+46 46 222 49 49


Sölvegatan 35, Lund


Research group

Plant Biology



Postdoc host

Olivier Van Aken