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Identification of Iridoid Glucoside Transporters in Catharanthus roseus

Author:
  • Lars Bo Larsen
  • Victoria L Fuller
  • Jacob Pollier
  • Alex Van Moerkercke
  • Fabian Schweizer
  • Richard J. Payne
  • Maite Colinas
  • Sarah E O'Connor
  • Alain Goossens
  • Barbara A Halkier
Publishing year: 2017-09-01
Language: English
Pages: 1507-1518
Publication/Series: Plant and Cell Physiology
Volume: 58
Issue: 9
Document type: Journal article
Publisher: Oxford University Press

Abstract english

Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.

Keywords

  • Biochemistry and Molecular Biology

Other

Published
  • ISSN: 1471-9053
Alex Van Moerkercke
E-mail: alex [dot] van_moerkercke [at] biol [dot] lu [dot] se

Postdoctoral fellow

Molecular Cell Biology

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B-A308

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Research group

Plant Biology

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Postdoc host

Olivier Van Aken