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Low Incubation Temperature Induces DNA Hypomethylation in Lizard Brains

Author:
  • Ursula Paredes
  • Reinder Radersma
  • Naomi Cannell
  • Geoffrey M. While
  • Tobias Uller
Publishing year: 2016-07-01
Language: English
Pages: 390-395
Publication/Series: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume: 325
Issue: 6
Document type: Journal article
Publisher: John Wiley and Sons Inc.

Abstract english

Developmental stress can have organizational effects on suites of physiological, morphological, and behavioral characteristics. In lizards, incubation temperature is perhaps the most significant environmental variable affecting embryonic development. Wall lizards (Podarcis muralis) recently introduced by humans from Italy to England experience stressfully cool incubation conditions, which we here show reduce growth and increase the incidence of scale malformations. Using a methylation-sensitive AFLP protocol optimized for vertebrates, we demonstrate that this low incubation temperature also causes hypomethylation of DNA in brain tissue. A consistent pattern across methylation-susceptible AFLP loci suggests that hypomethylation is a general response and not limited to certain CpG sites. The functional consequences of hypomethylation are unknown, but it could contribute to genome stability and regulation of gene expression. Further studies of the effects of incubation temperature on DNA methylation in ectotherm vertebrates may reveal mechanisms that explain why the embryonic thermal environment often has physiological and behavioral consequences for offspring.

Keywords

  • Zoology

Other

Published
  • ISSN: 1932-5223