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Services on Agilent BioAnalyzer

Agilent BioAnalyzer
Credits: Agilent Technologies All Rights Reserved

The Agilent 2100 BioAnalyzer system provides sizing, quantitation and quality control of DNA and RNA on a single platform, providing high quality digital data.

  • Ready-to-use assays and pre-packaged reagent kits
  • Minimal sample consumption (1-4 µL)
  • Digital data for convenient analysis, archiving and storage
  • Contamination-free switch of methods

Check the www.genomics.agilent.com for more details. For instance about salt requirements.

Applications

Many different applications are available for the 2100 BioAnalyzer but we only keep a few in stock (see below). Bring your samples in individual 1.7-ml Eppendorf tubes. Although just 1µl is needed of each sample please provide at least 3µl and whatever is left you get it back if samples are precious. Reagents for the following applications are usually kept in stock:

DNA

  • Agilent DNA 1000 Assay (12 samples/chip, 25–1000bp, 0.1-50ng/µl)
  • Agilent High Sensitivity DNA Assay (11 samples/chip, 50-7000bp, 5-500 pg/µl)

RNA

  • Agilent RNA 6000 Nano Total RNA Assay (12 samples/chip, 5-500ng/µl)

For any other application, please make an inquiry.

Forms

A Sample Form (xlsx; 13 kB) for analysis on Agilent BioAnalyzer is required containing information about the client, invoicing information and samples provided. Please label your tube(s) numerically in accordance. If more than one full-chip run is requested, one Sample Form for each chip should be provided.

Prices

For the Agilent DNA 1000 Assay and Agilent RNA 6000 Nano Total RNA Assay we charge 500 SEK/chip, whereas for the Agilent High Sensitivity DNA Assay we charge 600 SEK/chip. For all users “overhead” charges will be applied. For users outside Lund University we also need to charge for labor on an hourly basis.

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Analyzer equipment
Credits: Agilent Technologies All Rights Reserved

Contact information

The sequencing Facility is situated at Sölvegatan 37, in the Ecology Building on Floor 2 (stair case A)

For inquiries about Illumina MiSeq sequencing and analyses on Agilent BioAnalyzer contact:

Tomas Johansson
Researcher
MEMEG

Telephone: +46 46-222 45 49
Mobile: +46 709-57 18 04
E-mail: Tomas.Johansson [at] biol.lu.se

For inquiries about ABI Sanger sequencing contact:

Eva Friman
Research Engineer
MEMEG

Telephone: +46 46-222 08 70
Mobile: +46 767-64 52 66
E-mail: Eva.Friman [at] biol.lu.se

For inquiries about Bioinformatics contact:

Björn Canbäck
Research Engineer
MEMEG

Telephone: +46 46-222 94 19
E-mail: Bjorn.Canback [at] biol.lu.se

Funding

The facility acknowledges generous financial support during the years from the Knut and Alice Wallenberg Foundation (SWEGENE programme), the Crafoord Foundation and the Faculty of Science, Lund University.