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PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

Author:
  • Niklas Wahlberg
  • Carlos Peña
  • Milla Ahola
  • Christopher W. Wheat
  • Jadranka Rota
Publishing year: 2016
Language: English
Pages: 129-141
Publication/Series: ZooKeys
Volume: 2016
Issue: 596
Document type: Journal article
Publisher: Pensoft Publishers

Abstract english

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Keywords

  • Zoology
  • Biochemistry and Molecular Biology
  • Genetics
  • Lepidoptera
  • Molecular systematics
  • Phylogenetics
  • Phylogenomics

Other

Published
  • ISSN: 1313-2989
Professor Niklas Wahlberg at the unit of Biodiversity, Lund University.
E-mail: niklas.wahlberg [at] biol.lu.se

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